For DirectAccess deployments where domain controllers are running Windows Server 2003 or Windows Server 2003 R2 using the File Replication Service (FRS) for replication, DirectAccess client and server settings Group Policy Objects (GPOs) may be deleted. If these GPOs are deleted, DirectAccess connectivity will be disrupted. If the GPOs cannot be recovered via backup, it will be necessary to rebuild the entire DirectAccess deployment from scratch.

Microsoft recently updated their DirectAccess Unsupported Configurations documentation to reflect new guidance for DirectAccess deployments where the FRS is used for the distribution of Active Directory GPOs. DirectAccess is no longer supported in environments where FRS is used for SYSVOL replication.

What this means is that if you plan to deploy DirectAccess, domain controllers must be running Windows Server 2008 or later, and Distributed File System Replication (DFS-R) must be used for replication.

More details can be found here.